扩展莫尼茨绦虫wnt基因在虫体不同发育体节的差异表达

doi:10.11843/j.issn.0366-6964.2016.12.019

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (12): 2483-2492.doi: 10.11843/j.issn.0366-6964.2016.12.019

扩展莫尼茨绦虫wnt基因在虫体不同发育体节的差异表达

王正荣¹，张艳艳¹，薄新文^1*，徐雪平¹，徐春生²

（1.省部共建绵羊遗传改良与健康养殖国家重点实验室/新疆农垦科学院畜牧兽医研究所，石河子832000；2.石河子大学动物科技学院，石河子832000）

收稿日期:2016-04-27 出版日期:2016-12-23 发布日期:2016-12-23
通讯作者: 薄新文，男，研究员，主要从事寄生虫分子生物学研究，E-mail:851661263@qq.com
作者简介:王正荣(1984-)，男，助理研究员，主要从事寄生虫分子生物学研究，E-mail:wzrtiger@sina.com
基金资助:
国家重点基础研究发展计划(973计划）项目(2015CB150300）；国家自然科学基金(31360608）；新疆生产建设兵团科技攻关项目(2014BA002）；新疆生产建设兵团国际科技合作项目(2013BC001)；新疆生产建设兵团绵羊繁育生物技术重点实验室开放课题(2013KLS06）

Differential Expression of wnt Genes in Different Proglottides of Moniezia expansa

WANG Zheng-rong¹, ZHANG Yan-yan¹, BO Xin-wen^1* , XU Xue-ping¹, XU Chun-sheng²

(1. State Key Labaratory of Sheep Genetic Improvement and Healthy Production/Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China; 2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China)

Received:2016-04-27 Online:2016-12-23 Published:2016-12-23

摘要/Abstract

摘要：

研究扩展莫尼茨绦虫（Moniezia expansa）wnt基因家族wnt1、wnt2、wnt4、wnt5和wnt11B 5个基因在虫体不同发育体节的差异表达及组织分布规律，为进一步揭示Wnt信号通路在扩展莫尼茨绦虫发育中的作用奠定基础。作者采用SYBR GreenⅠqRT-PCR方法分析了wnt1、wnt2、wnt4、wnt5和wnt11B在虫体头颈节、幼节、成节和孕节的mRNA相对转录情况；采用核酸原位杂交方法分析上述5个基因在虫体头颈节、幼节、成节和孕节的mRNA分布情况。结果显示，wnt基因家族成员在虫体各发育体节均有表达，wnt1、wnt4、wnt5和wnt11B基因在虫体头颈节的mRNA转录水平相对较高，而在虫体孕节的mRNA转录水平最低；与之相反，wnt2基因在虫体头颈节的mRNA转录水平最低，而在虫体幼节的转录水平较高。原位杂交的结果表明，在虫体的头颈节，wnt基因主要表达于虫体的吸盘部位；在幼节和孕节，主要表达于虫体的节间腺，而在成节，wnt基因在虫体的雌雄生殖系统（如卵巢、睾丸）均有表达，并且在虫体的节间腺以及表皮也有一定的表达。综上，扩展莫尼茨绦虫5个wnt基因在虫体头颈节以及幼节的mRNA转录水平较高，且在虫体的吸盘、节间腺以及雌雄生殖细胞均有分布，因此初步推测Wnt信号通路可能参与扩展莫尼茨绦虫体节以及生殖细胞的发育过程，当然这一假说还有待进一步验证。

Abstract:

The mRNA relative expression and tissue distribution of wnt gene family wnt1, wnt2, wnt4, wnt5 and wnt11B in different proglottides of Moniezia expansa were investigated in the present study to provide basic information for exploring the roles of the wnt signaling pathway in development of M. expansa. The relative mRNA expression of wnt1, wnt2, wnt4, wnt5 and wnt11B in different proglottides of M. expansa were determined by qRT-PCR. Tissue location of wnt1, wnt2, wnt4, wnt5 and wnt11B in different proglottides of M. expansa were detected using in situ hybridization. The quantitative results showed that the gene family of wnt were transcribed in all proglottides of adult M. expansa. The transcription of wnt1, wnt4, wnt5 and wnt11B were higher in scolex than other proglottides, and in the gravid proglottides, the transcription of these genes was lowest. On the contrary, the transcription of wnt2 was lowest in the scolex, but higher in other proglottides. The in situ hybridization results showed that the wnt gene family mRNA were mainly distributed in suckers of scolex; in the mature proglottides, they were mainly distributed in ovary, testis and interproglottidal; in the immature and gravid proglottides, they were mainly distributed in interproglottidal. Based on these findings, the mRNA relative expression of these 5 wnt genes in scolex and immature proglottides was higher than in other proglottides, and mainly distributed in suckers, interproglottidal and germ cells. The results suggested that the Wnt signaling pathway may be involved in development of segments and germ cell in M. expansa, which remains to be further verified.

中图分类号:

S852.734

王正荣，张艳艳，薄新文，徐雪平，徐春生. 扩展莫尼茨绦虫wnt基因在虫体不同发育体节的差异表达[J]. 畜牧兽医学报, 2016, 47(12): 2483-2492.

WANG Zheng-rong, ZHANG Yan-yan, BO Xin-wen, XU Xue-ping, XU Chun-sheng. Differential Expression of wnt Genes in Different Proglottides of Moniezia expansa[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2483-2492.

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扩展莫尼茨绦虫wnt基因在虫体不同发育体节的差异表达

Differential Expression of wnt Genes in Different Proglottides of Moniezia expansa

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